Journal: Cells
Article Title: Estradiol Inhibits Human Brain Vascular Pericyte Migration Activity: A Functional and Transcriptomic Analysis
doi: 10.3390/cells10092314
Figure Lengend Snippet: Estradiol increases barrier function in endothelial cells (ECs) in presence of pericytes (PCs). Endothelial cells were grown on permeable transwell inserts alone ( a ) or in co-culture with PCs on the opposite site of the porous membrane ( b ). Cells were treated with estradiol (E2, 10 −8 M) or vehicle (Ctrl, DMSO) for 48 h and barrier function was measured by means of transendothelial electric resistance measurements with a cellZscope instrument. Experiments were performed 3 times in triplicates or duplicates and data represent mean +/− sd. ns p > 0.05, ** p < 0.01, compared to Ctrl.
Article Snippet: For TEER measurements in real-time, a cellZscope instrument (nanoAnalytics GmbH, Münster, Germany) was used.
Techniques: Co-Culture Assay, Membrane